NSE Antibody
Recognizes a protein of about 50kDa, which is identified as gamma-enolase/neuron specific enolase/enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Product Specifications
UniProt
P09104
Reactivity
Human
Immunogen
Amino acids 416-433 of human Neuron-specific Enolase were used as the immunogen for this NSE antibody.
Clonality
Monoclonal
Clone
NSEL1-1
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Protein G affinity chromatography
Dilution
Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 0.5-1ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Tested Applications
IHC-P, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG2b
Available Sizes
Frequently Asked Questions
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