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GRP94 Antibody / HSP90B1

Heat shock protein 90kDa beta member 1, known also as endoplasmin, gp96, grp94 and ERp99, is an HSP90 paralogue that is found in the endoplasmic reticulum. It plays critical roles in folding proteins in the secretory pathway such as Toll-like receptors and integrins. It has been implicated as an essential immune chaperone to regulate both innate and adaptive immunity. [Wiki]

Product Specifications

UniProt

P14625

Reactivity

Human

Immunogen

Recombinant full-length human HSP90B1 protein was used as the immunogen for the GRP94 antibody.

Clonality

Monoclonal

Clone

SRPR90b

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Protein G affinity chromatography

Dilution

Immunohistology (FFPE) : 1-2ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Titering of the GRP94 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P

Host or Source

Rat

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Rat IgG2a, kappa

Available Sizes

Frequently Asked Questions

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