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CD45RA Antibody

CD45RA is an isoform of the human leukocyte common antigen (CD45) . Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells.

Product Specifications

UniProt

P08575

Reactivity

Human

Immunogen

Recombinant human protein was used as immunogen for this anti-CD45RA antibody.

Clonality

Monoclonal

Clone

CDLA45RA-1

Conjugation

Unconjugated

Field of Research

Epigenetics, Infectious Diseases

Purification

Protein G affinity chromatography

Dilution

Western blot: 1-2ug/ml, Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 1-2 ug/ml, Immunohistology (FFPE) : 0.5-1.0ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Titering of the anti-CD45RA antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

FACS, IF, IHC, WB

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Mouse IgG2a, kappa

Available Sizes

Frequently Asked Questions

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