ER Antibody / Estrogen Receptor alpha
Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. ER presence in breast tumors indicates an increased likelihood of response to anti-estrogen therapy.
Product Specifications
Reactivity
Human
Immunogen
Recombinant human ER alpha protein (aa 2-185) was used as the immunogen.
Clonality
Monoclonal
Clone
NR3Ga-3
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Dilution
Western blot: 1-2ug/ml, Flow cytometry: 1-2ug/million cells, Immunohistochemistry (FFPE) : 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Variations in protocols, secondaries and substrates may require the ER antibody to be titered for optimal performance.
Tested Applications
FACS, IHC-P, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa
Available Sizes
Frequently Asked Questions
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