IgM Antibody
IgM is the first antibody generated in an immune response to an antigen. It is generally a pentamer with each of the five immunoglobulins linked together with disulfide bonds. In its pentamer form, it has a molecular mass of 970 kDa and 10 antigen binding sites (due to the large size of most antigens, not all binding sites can be filled simultaneously. IgM antibodies account for approximately 5%-10% of all the antibody in the body.
Product Specifications
Reactivity
Human
Immunogen
Human Mu heavy chain was used as the immunogen for this IgM antibody.
Clonality
Monoclonal
Clone
MuHC2
Conjugation
Unconjugated
Purification
Protein G
Dilution
Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Tested Applications
IHC-P, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa
Available Sizes
Frequently Asked Questions
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