ODC Antibody

Ornithine Decarboxylase (ODC) is the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. [RefSeq]

Product Specifications

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for this ODC antibody.

Clonality

Monoclonal

Clone

ORN255-1

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 1-2ug/million cells, Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Titering of the ODC antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.