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HCG alpha Antibody / Luteinizing Hormone alpha

This antibody reacts with a protein of ~13kDa, identified as alpha sub-unit of hCG. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. HCG is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH) .

Product Specifications

UniProt

P01215

Reactivity

Human

Immunogen

Recombinant protein was used as the immunogen for this hCG alpha antibody.

Clonality

Monoclonal

Clone

CGA91-2

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Protein G purified monoclonal antibody

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Variations in protocols, secondaries and substrates may require the hCG alpha antibody to be titered for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Mouse IgG1, kappa

Available Sizes

Frequently Asked Questions

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