Glial Fibrillary Acidic Protein Antibody / GFAP
This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP) . It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Product Specifications
UniProt
P14136
Reactivity
Human
Immunogen
A portion of amino acids 101-200 were used as the immunogen for the Glial Fibrillary Acidic Protein antibody.
Clonality
Monoclonal
Clone
GFAP/2076
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Protein G affinity chromatography
Dilution
Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Western blot: 2-4ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the Glial Fibrillary Acidic Protein antibody should be determined by the researcher.
Tested Applications
FACS, IHC-P, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa
Available Sizes
Frequently Asked Questions
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