Recombinant CD99 Antibody / Rabbit Monoclonal

CD99 is found on the cell surface of Ewing's sarcoma tumors and is positive in granulosa cell tumors. It is more expressed in malignant gliomas than in the brain, and such overexpression results in higher levels of invasiveness and lower rates of survival. Antibodies to CD99 are used in diagnostic immunohistochemistry to distinguish Ewing's sarcoma from other tumours of similar histological appearance, as well as for the identification of thymic tumours, and of spindle cell tumours, such as synovial sarcoma, haemangiopericytoma, and meningioma.[Wiki]

Product Specifications

UniProt

P14209

Reactivity

Human

Immunogen

Human protein was used as the immunogen for the recombinant CD99 antibody.

Clonality

Recombinant

Clone

CDLA99-2R

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein A affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 5-10ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant CD99 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.