Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Recombinant EpCAM Antibody

Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Product Specifications

UniProt

P16422

Reactivity

Human

Immunogen

Neuraminidase treated GLS-1 human small cell lung carcinoma cells were used as the immunogen for the recombinant EpCAM antibody. Binding epitope of the rMOC-31 antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59.

Clonality

Recombinant

Clone

RMOC-31

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT, Immunofluorescence: 1-2ug/ml, Western blot: 1-2ug/ml, Flow cytometry: 1-2ug/million cells

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

FACS, IF, IHC-P, WB

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Mouse IgG1, kappa

Available Sizes

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

Replication Protein A2 (RPA2) (RPA2/2106), CF640R conjugate, 0.1mg/mL
BNC402106-500 1x 500 µL

Replication Protein A2 (RPA2) (RPA2/2106), CF640R conjugate, 0.1mg/mL

Sign In for Pricing
View Details
Thromboxane A2 receptor (TBXA2R) (NM_001060) Human Over-expression Lysate
LY420740 100 µg

Thromboxane A2 receptor (TBXA2R) (NM_001060) Human Over-expression Lysate

Sign In for Pricing
View Details
Ufm1 Mouse Gene Knockout Kit (CRISPR)
KN518655 1 Kit

Ufm1 Mouse Gene Knockout Kit (CRISPR)

Sign In for Pricing
View Details
SDHAF1 Mouse Monoclonal Antibody [Clone ID: OTI2G3]
CF809734 100 µg

SDHAF1 Mouse Monoclonal Antibody [Clone ID: OTI2G3]

Sign In for Pricing
View Details
Nicotinic Acetylcholine Receptor alpha 7 (CHRNA7) Human Gene Knockout Kit (CRISPR)
KN421382 1 Kit

Nicotinic Acetylcholine Receptor alpha 7 (CHRNA7) Human Gene Knockout Kit (CRISPR)

Sign In for Pricing
View Details
CD68 (C68/684), CF647 conjugate, 0.1mg/mL
BNC470684-100 1x 100 µL

CD68 (C68/684), CF647 conjugate, 0.1mg/mL

Sign In for Pricing
View Details