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Spectrin beta III Antibody / SPTBN2

Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Spectrin alpha and spectrin beta are present in erythrocytes, whereas spectrin alpha II (fodrin a) and spectrin beta I (fodrin b) are present in other somatic cells. SPTBN2/Spectrin beta III is highly homologous to both spectrin beta I and spectrin beta II. SPTBN2 is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta.

Product Specifications

UniProt

O15020

Reactivity

Human

Immunogen

Human recombinant partial protein corresponding to amino acids 356-475 was used as the immunogen for this Spectrin beta III antibody.

Clonality

Monoclonal

Clone

SPTBN2/1582

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Protein G affinity chromatography

Dilution

ELISA: 2-4ug/ml (order BSA/azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 0.5-1ug/ml, Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Spectrin beta III antibody to be titered up or down for optimal performance.

Tested Applications

ELISA, FACS, IF, IHC-P, WB

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Mouse IgG2a, kappa

Available Sizes

Frequently Asked Questions

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