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E. coli O157 Antibody

This mAb shows specificity to E. coli O157 in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms) . Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56) . The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following four groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC) . Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.

Product Specifications

Reactivity

E. coli

Immunogen

A crude sonicate of E. coli O157 was used as the immunogen for the E. coli O157 antibody.

Clonality

Monoclonal

Clone

9.88

Conjugation

Unconjugated

Field of Research

Infectious Disease & Virology

Purification

PEG precipitation followed by dialysis in 1X PBS

Dilution

ELISA: 1-5ug/ml for coating (order BSA/sodium azide-free format), Immunofluorescence: 1-2ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the E. coli O157 antibody should be determined by the researcher.

Tested Applications

ELISA, IF

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Mouse IgM, kappa

Available Sizes

Frequently Asked Questions

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