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Rat EpCAM Antibody

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.

Product Specifications

UniProt

O55159

Reactivity

Mouse, Rat

Immunogen

Recombinant full-length rat protein was used as the immunogen for the rat EpCAM antibody.

Clonality

Monoclonal

Clone

Epcam/1158

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Unpurified high titer supernatant

Dilution

Immunohistochemistry (FFPE) : 1:50-1:100 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (3)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the rat EpCAM antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The epitopes of mAbs Epcam/1158 and Epcam/1159 monoclonal antibodies are different.3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

Bioreactor concentrate with 0.05% sodium azide

Isotype

Mouse IgG1, kappa

Available Sizes

Frequently Asked Questions

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