CDC2 Antibody / CDK1
Recognizes a 34kDa protein, which is identified as cyclin dependent kinase 1 (cdk1) or p34cdc2 protein kinase. Cdc2 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated Cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.
Product Specifications
UniProt
P06493
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the CDC2 antibody.
Clonality
Monoclonal
Clone
CDK1/873
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Protein G affinity chromatography
Dilution
Immunohistochemistry (FFPE) : 2-4ug/ml for 30 min at RT, Western blot: 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the CDC2 antibody should be determined by the researcher.
Tested Applications
IHC-P, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG2a, kappa
Available Sizes
Frequently Asked Questions
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