CD1b Antibody
The mouse monoclonal antibody recognizes CD1b, a 44kDa type I glycoprotein associated with beta2-microglobulin (Workshop IV; Code T015) . It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells. The CD1 multigene family encodes five forms of the CD1 T-cell surface glycoprotein in human, designated CD1A, 1B, 1C, 1D and 1E. CD1, a type 1 membrane protein, has structural similarity to the MHC class I antigen and has been shown to present lipid antigens for recognition by T lymphocytes. Constitutive endocytosis of CD1B molecules and the differential sorting of MHC class II from lysosomes separate peptide- and lipid antigen-presenting molecules during dendritic cell maturation. CD1B is also expressed in interdigitating cells.
Product Specifications
UniProt
P29016
Reactivity
Human
Immunogen
Stimulated human leukocytes were used as the immunogen for the CD1b antibody.
Clonality
Monoclonal
Clone
100-1A5
Conjugation
Unconjugated
Field of Research
Epigenetics, Immunology
Purification
PEG precipitation
Dilution
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1ug/ml, Functional studies (order BSA/sodium azide-free format)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the CD1b antibody should be determined by the researcher.
Tested Applications
FACS, Functional Studies, IF
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgM, kappa
Available Sizes
Frequently Asked Questions
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