What is the catalog number for ZAP-70 Antibody?

The catalog number for ZAP-70 Antibody is orb749850.

What sizes are available for ZAP-70 Antibody?

ZAP-70 Antibody is available in the following size(s): 20 µg, 100 µg.

How many pack sizes does ZAP-70 Antibody come in?

ZAP-70 Antibody is available in 2 different pack sizes.

Is ZAP-70 Antibody in stock?

ZAP-70 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

ZAP-70 Antibody

ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.

Product Specifications

UniProt

P43403

Reactivity

Human

Immunogen

Recombinant human protein (including amino acids 1-254 and the SH2 domains) was used as the immunogen for the ZAP-70 antibody.

Clonality

Monoclonal

Clone

2F3.2

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Protein G affinity chromatography

Dilution

Western blot: 1-2ug/ml, Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the ZAP-70 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.