What is the catalog number for Vimentin Antibody?

The catalog number for Vimentin Antibody is orb749843.

What sizes are available for Vimentin Antibody?

Vimentin Antibody is available in the following size(s): 20 µg, 100 µg.

How many pack sizes does Vimentin Antibody come in?

Vimentin Antibody is available in 2 different pack sizes.

Is Vimentin Antibody in stock?

Vimentin Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Vimentin Antibody

This mAb reacts with a 58kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin. Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.

Product Specifications

UniProt

P08670

Reactivity

Human, Mouse, Rat

Immunogen

A human thymic nuclear extract was used as the immunogen for the Vimentin antibody.

Clonality

Monoclonal

Clone

LN-6

Conjugation

Unconjugated

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

PEG precipitation

Dilution

Immunohistochemistry (FFPE) : 0.1-0.2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the Vimentin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.