What is the catalog number for Fascin Antibody?

The catalog number for Fascin Antibody is orb749791.

What sizes are available for Fascin Antibody?

Fascin Antibody is available in the following size(s): 20 µg, 100 µg.

How many pack sizes does Fascin Antibody come in?

Fascin Antibody is available in 2 different pack sizes.

Is Fascin Antibody in stock?

Fascin Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Fascin Antibody

Fascin organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. [UniProt]

Product Specifications

UniProt

Q16658

Reactivity

Human

Immunogen

Full length recombinant human protein was used as the immunogen for the Fascin antibody.

Clonality

Monoclonal

Clone

SPM133

Conjugation

Unconjugated

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Protein G affinity chromatography

Dilution

Western blot: 1-2ug/ml, Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the Fascin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.