What is the catalog number for Podocalyxin Antibody?

The catalog number for Podocalyxin Antibody is orb749747.

What sizes are available for Podocalyxin Antibody?

Podocalyxin Antibody is available in the following size(s): 20 µg, 100 µg.

How many pack sizes does Podocalyxin Antibody come in?

Podocalyxin Antibody is available in 2 different pack sizes.

Is Podocalyxin Antibody in stock?

Podocalyxin Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Podocalyxin Antibody

Podocalyxin/PODXL is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Product Specifications

UniProt

O00592

Reactivity

Human, Rat

Immunogen

A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human PODXL was used as the immunogen for the Podocalyxin antibody.

Clonality

Monoclonal

Clone

2A4

Conjugation

Unconjugated

Field of Research

Epigenetics

Purification

PEG precipitation

Dilution

Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT, Western blot: 1-2ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the Podocalyxin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.