What is the catalog number for EpCAM Antibody / Cytoplasmic domain?

The catalog number for EpCAM Antibody / Cytoplasmic domain is orb749649.

What sizes are available for EpCAM Antibody / Cytoplasmic domain?

EpCAM Antibody / Cytoplasmic domain is available in the following size(s): 20 µg, 100 µg.

How many pack sizes does EpCAM Antibody / Cytoplasmic domain come in?

EpCAM Antibody / Cytoplasmic domain is available in 2 different pack sizes.

Is EpCAM Antibody / Cytoplasmic domain in stock?

EpCAM Antibody / Cytoplasmic domain is currently made to order / not held in immediate stock. Contact us for lead time.

EpCAM Antibody / Cytoplasmic domain

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Product Specifications

UniProt

P16422

Reactivity

Canine, Feline, Human, Mouse, Rat

Immunogen

A portion of amino acids 289-314 (cytoplasmic domain) was used as the immunogen for the EpCAM antibody.

Clonality

Monoclonal

Clone

EGP40/1110

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the EpCAM antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.