What is the catalog number for ICAM3 Antibody?

The catalog number for ICAM3 Antibody is orb749584.

What sizes are available for ICAM3 Antibody?

ICAM3 Antibody is available in the following size(s): 20 µg, 100 µg.

How many pack sizes does ICAM3 Antibody come in?

ICAM3 Antibody is available in 2 different pack sizes.

Is ICAM3 Antibody in stock?

ICAM3 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

ICAM3 Antibody

Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb is excellent for staining of formalin/paraffin tissues.

Product Specifications

UniProt

P32942

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the ICAM3 antibody.

Clonality

Monoclonal

Clone

ICAM3/1019

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Protein G affinity chromatography

Dilution

Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the ICAM3 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.