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HLA-DPB1 Antibody (MHC II)

Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35kDa) and beta (27-30kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders.

Product Specifications

UniProt

P04440

Reactivity

Human

Immunogen

Non-T, non-B human acute lymphoblastic leukemia REH6 cell line was used as the immunogen for the HLA-DPB1 antibody.

Clonality

Monoclonal

Clone

BraFB6

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Protein G affinity chromatography

Dilution

ELISA: 2-4ug/ml for coating (order BSA/sodium azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the HLA-DPB1 antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Tested Applications

ELISA, FACS, IF

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Mouse IgG2b, kappa

Available Sizes

Frequently Asked Questions

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