What is the catalog number for GFAP Antibody?

The catalog number for GFAP Antibody is orb749517.

What sizes are available for GFAP Antibody?

GFAP Antibody is available in the following size(s): 20 µg, 100 µg.

How many pack sizes does GFAP Antibody come in?

GFAP Antibody is available in 2 different pack sizes.

Is GFAP Antibody in stock?

GFAP Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

GFAP Antibody

This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP) . It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Product Specifications

UniProt

P14136

Reactivity

Human, Rat

Immunogen

Full length recombinant protein was used as the immunogen for the GFAP antibody.

Clonality

Monoclonal

Clone

ASTRO/789

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Protein G affinity chromatography

Dilution

Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the GFAP antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.