CD15 Antibody
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on > 95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
Product Specifications
UniProt
P22083
Reactivity
Human
Immunogen
Myelomonocytic leukemia cells were used as the immunogen for the CD15 antibody.
Clonality
Monoclonal
Clone
BRA-4F1
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
PEG precipitation
Dilution
Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 0.5-1ug/ml, Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Tested Applications
FACS, IF, IHC-P
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgM, kappa
Available Sizes
Frequently Asked Questions
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