Semaphorin-4D Antibody / SEMA4D / CD100
Recognizes a homodimeric protein comprised of 50kDa subunits, identified as CD100. It is expressed on majority of haemopoietic cells (B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. Its expression is increased after PHA-activation. CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and a Serine kinase. It plays a role in homotypic cell adhesion and in T cell activation.
Product Specifications
UniProt
Q92854
Reactivity
Human
Immunogen
Human recombinant protein was used as the immunogen for the Semaphorin-4D antibody.
Clonality
Monoclonal
Clone
SEMA4D/933
Conjugation
Unconjugated
Field of Research
Neuroscience; Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the Semaphorin-4D antibody should be determined by the researcher.
Tested Applications
FACS, IF
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa
Available Sizes
Frequently Asked Questions
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