Semaphorin-4D Antibody / SEMA4D / CD100
Recognizes a homodimeric protein comprised of 50kDa subunits, identified as Semaphorin-4D, or CD100. It is expressed on majority of haemopoietic cells (B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. Its expression is increased after PHA-activation. CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and a Serine kinase. It plays a role in homotypic cell adhesion and in T cell activation.
Product Specifications
UniProt
Q92854
Reactivity
Human, Mouse
Immunogen
PHA stimulated human peripheral blood lymphocytes were used as the immunogen for the Semaphorin-4D antibody.
Clonality
Monoclonal
Clone
A8
Conjugation
Unconjugated
Field of Research
Neuroscience; Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
Functional studies (order BSA/sodium azide-free format), Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the Semaphorin-4D antibody to be determined by the researcher.
Tested Applications
FACS, Functional Studies, IF
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa
Available Sizes
Frequently Asked Questions
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