P27 Antibody

Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This mAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.

Product Specifications

UniProt

P46527

Reactivity

Human, Mouse, Rat

Immunogen

Mouse recombinant protein was used as the immunogen for the p27 antibody. The epitope has been localized to amino acids 83-204.

Clonality

Monoclonal

Clone

DCS-72.F6

Conjugation

Unconjugated

Field of Research

Cancer, Epigenetics

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1ug/ml, Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the p27 antibody to be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.