CD45RO Antibody Cocktail

Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) . This antibody cocktail reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

Product Specifications

UniProt

P08575

Reactivity

Human

Immunogen

Cultured human T-cells from an IL-2-dependent T-cell line (CA1) (clone UCHL-1) and human T lymphocytes (clone T200/797) were used as the immunogens for this CD45RO antibody cocktail.

Clonality

Monoclonal

Clone

UCHL-1 + T200/797

Conjugation

Unconjugated

Field of Research

Epigenetics, Infectious Diseases

Purification

Protein G affinity chromatography

Dilution

Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Due to variation in protocol and secondary antibody used, the CD45RO antibody cocktail may need to be titered for optimal performance.1. FFPE staining requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.