CD45RO Antibody
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) . This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Product Specifications
UniProt
P08575
Reactivity
Human
Immunogen
Human T lymphocytes were used as immunogen for this CD45RO antibody.
Clonality
Monoclonal
Clone
T200/797
Conjugation
Unconjugated
Field of Research
Epigenetics, Infectious Diseases
Purification
Protein G affinity chromatography
Dilution
Flow cytometry: 0.5-1ug/10^6 cells, Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Tested Applications
FACS, IHC-P
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG2a, kappa
Available Sizes
Frequently Asked Questions
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