What is the catalog number for EMA Antibody / MUC1 / Mucin-1?

The catalog number for EMA Antibody / MUC1 / Mucin-1 is orb385659.

What sizes are available for EMA Antibody / MUC1 / Mucin-1?

EMA Antibody / MUC1 / Mucin-1 is available in the following size(s): 20 µg, 100 µg.

How many pack sizes does EMA Antibody / MUC1 / Mucin-1 come in?

EMA Antibody / MUC1 / Mucin-1 is available in 2 different pack sizes.

Is EMA Antibody / MUC1 / Mucin-1 in stock?

EMA Antibody / MUC1 / Mucin-1 is currently made to order / not held in immediate stock. Contact us for lead time.

EMA Antibody / MUC1 / Mucin-1

This antibody recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of MUC1 (Mucin-1) or EMA (epithelial membrane antigen) . The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. MUC1 / Mucin-1 may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling through phosphorylations and protein-protein interactions. In immunohistochemical assays, the MUC1 / EMA antibody superbly stains routine formalin/paraffin carcinoma tissues. MUC1 antibody is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Product Specifications

Reactivity

Human

Immunogen

Human milk fat globule membranes were used as the immunogen for this EMA antibody.

Clonality

Monoclonal

Clone

MUC1/845

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EMA antibody to be titered up or down for optimal performance.1. HIER: boil sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 10-20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.