What is the catalog number for Tyrosinase Antibody?

The catalog number for Tyrosinase Antibody is orb248583.

What sizes are available for Tyrosinase Antibody?

Tyrosinase Antibody is available in the following size(s): 20 µg, 100 µg.

How many pack sizes does Tyrosinase Antibody come in?

Tyrosinase Antibody is available in 2 different pack sizes.

Is Tyrosinase Antibody in stock?

Tyrosinase Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Tyrosinase Antibody

Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients.

Product Specifications

Reactivity

Human

Immunogen

Recombinant Tyrosinase protein was used as the immunogen for this antibody. Subsequent testing has localized the epitope to within amino acids 233-247 (IPYWDWRDAEKCDIC) (see Citation 12) .

Clonality

Monoclonal

Clone

T311

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.