What is the catalog number for PCNA Antibody?

The catalog number for PCNA Antibody is orb749379.

What sizes are available for PCNA Antibody?

PCNA Antibody is available in the following size(s): 20 µg, 100 µg.

How many pack sizes does PCNA Antibody come in?

PCNA Antibody is available in 2 different pack sizes.

Is PCNA Antibody in stock?

PCNA Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

PCNA Antibody

This antibody recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA) . PCNA is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA-cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This antibody is excellent for the detection of PCNA in multiple applications.

Product Specifications

Reactivity

Human, Mouse, Rat

Immunogen

Rat PCNA / Protein A fusion protein was used as the immunogen for this antibody.

Clonality

Monoclonal

Clone

PC10

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 0.5-1ug/ml, Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.