What is the catalog number for Kappa Light Chain Antibody?

The catalog number for Kappa Light Chain Antibody is orb248396.

What sizes are available for Kappa Light Chain Antibody?

Kappa Light Chain Antibody is available in the following size(s): 20 µg, 100 µg.

How many pack sizes does Kappa Light Chain Antibody come in?

Kappa Light Chain Antibody is available in 2 different pack sizes.

Is Kappa Light Chain Antibody in stock?

Kappa Light Chain Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Kappa Light Chain Antibody

This antibody is specific to the kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. In general, the ratio of Kappa to Lambda is 3:1. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Product Specifications

Reactivity

Human

Immunogen

Human B-Lymphoma Cells were used as the immunogen for this Kappa Light Chain antibody.

Clonality

Monoclonal

Clone

L1C1

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G affinity chromatography

Dilution

Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.