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IgG Antibody

This antibody recognizes a protein of 75kDa, identified as the gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. The antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Product Specifications

Reactivity

Human

Immunogen

Purified human IgG heavy chain was used as the immunogen for this anti-IgG antibody.

Clonality

Monoclonal

Clone

ICO-97

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

ELISA (order BSA/sodium azide-free format for coating), Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-IgG antibody to be titered up or down for optimal performance.

Tested Applications

ELISA, FACS, IF

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Mouse IgG1, kappa

Available Sizes

Frequently Asked Questions

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