What is the catalog number for HSP27 Antibody?

The catalog number for HSP27 Antibody is orb248376.

What sizes are available for HSP27 Antibody?

HSP27 Antibody is available in the following size(s): 20 µg, 100 µg.

How many pack sizes does HSP27 Antibody come in?

HSP27 Antibody is available in 2 different pack sizes.

Is HSP27 Antibody in stock?

HSP27 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

HSP27 Antibody

This antibody recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27) . HSP27 was recently found to be identical to the estrogen-induced 'p29' and '24K' protein. About 50% of breast carcinomas are positive for HSP27, especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.

Product Specifications

UniProt

P04792

Reactivity

Human, Mouse, Rat

Immunogen

Partially purified human HSP27 (earlier called 24K) from breast cancer MCF-7 cells was used as the immunogen for this antibody.

Clonality

Monoclonal

Clone

G3.1

Conjugation

Unconjugated

Field of Research

Epigenetics, Infectious Diseases, Neuroscience

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.