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EGF Receptor Antibody

This antibody reacts with the extracellular domain of EGF receptor and blocks the EGF/TGFa-induced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGF receptor.EGFR is type I receptor tyrosine kinase with sequence homology to ErbB2, -3 -4 or HER2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGFa), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin.

Product Specifications

Reactivity

Human

Immunogen

Microsomes from A431 cells were used as the immunogen for this antibody.

Clonality

Monoclonal

Clone

B1D8

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Protein G purified monoclonal antibody

Dilution

Affinity purification (order BSA/sodium azide-free format for coupling), Blocks ligand-induced activation of EGFR, Inhibits proliferation of A431 cells (order BSA/azide-free format), Arrests tumor growth in vivo (order BSA/azide-free format), Immunoprecipitation: 1-2ug/500ug protein lysate, Flow cytometry: 0.5-1ug/10^6 cells

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Tested Applications

Blocking, FACS, IP, NeA, Purification

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Mouse IgG2a, kappa

Available Sizes

Frequently Asked Questions

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