What is the catalog number for P27Kip1 Antibody?

The catalog number for P27Kip1 Antibody is orb248293.

What sizes are available for P27Kip1 Antibody?

P27Kip1 Antibody is available in the following size(s): 20 µg, 100 µg.

How many pack sizes does P27Kip1 Antibody come in?

P27Kip1 Antibody is available in 2 different pack sizes.

Is P27Kip1 Antibody in stock?

P27Kip1 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

P27Kip1 Antibody

This antibody recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. The p27Kip1 antibody is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. It is a candidate tumor suppressor gene. This mAb is excellent for staining of formalin-fixed tissues.

Product Specifications

Reactivity

Human, Mouse, Rat

Immunogen

Purified GST-p27 fusion protein of human origin was used as the immunogen for this p27Kip1 antibody.

Clonality

Monoclonal

Clone

SX53G8

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 0.5-1ug/ml, Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the p27Kip1 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.