S100A8 Antibody
The S100 family of proteins are found in vertebrates and are characterized by two calcium-binding sites that have a helix-loop-helix conformation. The family of 24 is divided into three subgroups based on function: those with intracellular, intracellular plus extracellular, and extracellular regulatory effects. The calcium-binding protein S100A8, also called MRP8 or Myeloid-related protein 8, and Calgranulin A, forms a heterodimer with S100A9, called Calprotectin. The heterodimer is expressed by granulocytes, monocytes, and early differentiation states of macrophages, and is known to be an important pro-inflammatory mediator in acute and chronic inflammation. S100A8 and 9 are DAMPs, or Damage-associated molecular pattern molecules, and are able to activate receptors of the innate immune system, one being TLR4.
Product Specifications
Reactivity
Human
Immunogen
A recombinant protein fragment from the N-terminal region of human S100A8 was used as the immunogen for this antibody.
Clonality
Monoclonal
Clone
SA6279
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Protein G affinity chromatography
Dilution
Western blot: 2-4ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Provided assay concentrations are suggestions only, S100A8 antibody titration may be required for optimal results.
Tested Applications
WB
Host or Source
Mouse
Preservative
0.5 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Isotype
Mouse IgG1, kappa
Available Sizes
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