ICAD Antibody
During apoptosis, toxic or useless cells are broken down for removal. DFF, or DNA fragmentation factor, is a heterodimeric protein which mediates DNA fragmentation and chromatin condensation during apoptosis. DFFB is the active DFF subunit but remains inactive while bound to DFFA, also call ICAD (Inhibitor of caspase-activated DNase) . Caspase-3 cleaves ICAD, which then disassociates from DFFB. The disassociation removes the inhibitory effects of ICAD, activating DFFB and beginning the DNA fragmentation process.
Product Specifications
Reactivity
Mouse
Immunogen
A recombinant protein fragment from human ICAD was used as the immunogen for this antibody.
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Protein A affinity chromatography
Dilution
Western blot: 4-8ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Provided assay concentrations are suggestions only, antibody titration may be required for optimal results.
Tested Applications
WB
Host or Source
Rabbit
Preservative
0.5 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Isotype
Rabbit IgG
Available Sizes
Frequently Asked Questions
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