GAPDH Antibody Loading Control
Due to its ubiquitous presence in mammalian cells, GAPDH is often used a loading control protein in western blot. GAPDH antibody is used to control for/demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.
Product Specifications
Reactivity
Canine, Human, Mouse, Primate, Rat
Immunogen
A recombinant protein fragment from C-terminal GAPDH was used as the immunogen for the GAPDH antibody.
Clonality
Monoclonal
Clone
GAP259
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Protein G Affinity Chromatography
Dilution
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE) : 3-5ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Provided assay concentrations are suggestions only, GAPDH antibody titration may be required for optimal results.
Tested Applications
IHC-P, WB
Host or Source
Mouse
Preservative
0.5 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Isotype
Mouse IgG1, kappa
Available Sizes
Frequently Asked Questions
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