Anti-Survivin (NT) Antibody

Rabbit Polyclonal Antibody specific to Survivin (NT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Apoptosis inhibitor 4, Apoptosis inhibitor survivin

Gene Name

BIRC5

Gene ID

332

NCBI Gene ID

UniProt

O15392

Accession Number

NP_001159

Cellular Locus

Cytoplasm, Nucleus, Chromosome, Chromosome, centromere, Cytoplasm, cytoskeleton, spindle, Chromosome, centromere, kinetochore, Midbody

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to aa 1-12 of human Survivin (accession no. NP_001159).

Target Antigen

Baculoviral IAP repeat-containing protein 5

Target

Survivin (NT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC, IHC-P, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:9859993, PubMed:21364656, PubMed:20627126, PubMed:25778398, PubMed:28218735). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255). May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993). The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784). May play a role in neoplasia (PubMed:10626797). Inhibitor of CASP3 and CASP7 (PubMed:21536684). Essential for the maintenance of mitochondrial integrity and function (PubMed:25778398). Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752). Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797). {PubMed:10626797, PubMed:12773388, PubMed:16291752, PubMed:16322459, PubMed:18591255, PubMed:20627126, PubMed:20826784, PubMed:20929775, PubMed:21364656, PubMed:21536684, PubMed:25778398, PubMed:28218735, PubMed:9859993}.

Additionnal Information

Immunoblotting: use at 1ug/mL. Positive control: MOLT4 cell lysate. Immunocytochemistry: use at 5ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human Survivin (17kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Apoptosis can be prevented by the inhibitor of apoptosis proteins (IAPs). IAPs constitute a conserved gene family that binds to and inhibits cell death proteases. A novel IAP has been identified and designated Survivin. Survivin interacts with the processed form of caspase-3 and inhibits its proteolytic activity. Survivin is expressed predominantly in tissues of embryos, transformed cell lines, and many human cancers.