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Beta Tubulin Antibody Loading Control

Selected Variant

GTR18080792-01

Size: 25 µg

There are five mammalian members of the tubulin family: alpha, beta, gamma, delta, and epsilon. Heterodimers of alpha and beta tubulin make up microtubules, which along with microfilaments and intermediate filaments, make up the cytoskeleton. Due to its ubiquitous presence in mammalian cells, it is often used a loading control protein in western blot. An antibody to beta-tubulin is used to demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.

Product Specifications

Reactivity

Canine, Human, Mouse, Primate, Rat

Immunogen

A recombinant protein fragment from the C-terminal region of human beta tubulin was used as the immunogen for this antibody.

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Protein A Affinity Chromatography

Dilution

Western blot: 2-4ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Provided assay concentrations are suggestions only, antibody titration may be required for optimal results.

Tested Applications

WB

Host or Source

Rabbit

Preservative

0.5 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide

Isotype

Rabbit IgG

Available Sizes

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