Beta Actin Antibody Loading Control
Actins proteins are highly conserved and involved in cellular structure, integrity and motility. There are three main isoforms of actin in vertebrates: alpha, beta and gamma. Alpha actins are found in muscle cells while beta and gamma actins are non-muscle actins. Beta actin is commonly used as a loading control protein in western blot. An antibody to beta actin is used to demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.
Product Specifications
Reactivity
Canine, Gallus, Human, Monkey, Mouse, Rabbit, Rat
Immunogen
A recombinant protein fragment from the N-terminus of human beta Actin was used as the immunogen for this antibody.
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Protein A Affinity Chromatography
Dilution
Western blot: 1-3ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Provided assay concentrations are suggestions only, beta Actin antibody titration may be required for optimal results.
Tested Applications
WB
Host or Source
Rabbit
Preservative
0.5 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Isotype
Rabbit IgG
Available Sizes
Frequently Asked Questions
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