IKKb Antibody
NF-kB is sequestered in the cytoplasm by IkBa, unable to translocate to the nucleus. IkB is part of the canonical pathway for NF-kB activation. IKKb is part of the IkB kinase complex, along with a second catalytic subunit, IKKa, and a regulatory subunit, IKKg. Along with IKKa, IKKb phosphorylates IkBa leading to its degradation and release of inhibition of NF-kB. The RELA subunit of active NF-kB is then free to enter the nucleus and initiate a number of signaling cascades.
Product Specifications
Reactivity
Human, Mouse
Immunogen
A recombinant protein fragment of IKKb was used as the immunogen for this antibody.
Clonality
Monoclonal
Clone
IKB355
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Protein G purified antibody
Dilution
Western blot: 2-4ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Provided assay concentrations are suggestions only, IKKb antibody titration may be required for optimal results.
Tested Applications
WB
Host or Source
Mouse
Preservative
0.5 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Isotype
Mouse IgG1, kappa
Available Sizes
Frequently Asked Questions
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