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GIT1 Antibody

Mouse Anti-Rat GIT1 Monoclonal IgG1

Product Specifications

Product Name Alternative

ARF GAP GIT1, ARF GTPase-activating protein, ARF GTPase-activating protein GIT1, CAT1, Cool-associated and tyrosine-phosphorylated protein 1, G protein-coupled receptor kinase-interactor 1, GIT 1, GIT1, GRK-interacting protein 1

UniProt

Q9Z272

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein amino acids 375-770 (C-terminus) of rat GIT1

Target

GIT1

Clonality

Monoclonal

Clone

N39B/8 (Formerly sold as S39B-8)

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Protein G Purified

Concentration

1 mg/ml

Dilution

WB (1:1000)

Molecular Weight

90kDa

References & Citations

Cason, Sydney E. et al. Axonal transport of autophagosomes is regulated by dynein activators JIP3/JIP4 and ARF/RAB GTPases bioRxiv, (2023)

Pubmed id

36747648

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

1 μg/ml was sufficient for detection of GIT1 in 10 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Tested Applications

ICC, IF, IP, WB

NCBI Accession Number

NP_114002.1

Host or Source

Mouse

Preservative

PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.

Isotype

IgG1

Entrez

83709

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