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4-Hydroxynonenal Antibody

Mouse Anti-4-Hydroxynonenal (4-HNE) Monoclonal IgG1

Product Specifications

Product Name Alternative

4-Hydroxynonenal, 4-Hydroxy-2-nonenal, 4-HNE, HNE, (2E) -4-Hydroxy-2-nonenal, 4-hydroxynon-2-enal, 2-Nonenal, 4-hydroxy-, 4-Hydroxy-2,3-nonenal, 4-hydroxylnonenal, non-2-enal, 4-hydroxy-

Reactivity

All

Immunogen

Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH) .

Target

4-Hydroxynonenal

Clonality

Monoclonal

Clone

12F7

Conjugation

Unconjugated

Field of Research

Cancer Biology, Cell Biology, Metabolism Research

Purification

Protein G Purified

Concentration

1 mg/ml

Dilution

WB (1:1000) ; ICC/IF (1:50) ; ELISA (1:1000)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

A 1:1000 dilution was sufficient for detection of 4-Hydroxynonenal in 0.5 μg of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Tested Applications

ELISA, ICC, IF, WB

Host or Source

Mouse

Preservative

PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.

Isotype

IgG1

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