CD99 Antibody / MIC2

CD99 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32) . It is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P14209

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-185) from the human protein was used as the immunogen for the CD99 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

MIC2/7862

Applications

WB, IHC-P

Purity

Protein A affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the CD99 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CD99 antibody is available for research use only.

Storage Conditions

Aliquot the CD99 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CD99 antibody should be determined by the researcher.

Location

Cell surface

Image Legend

Western blot testing of human MCF-7 cell lysate with CD99 antibody (clone MIC2/7862) . Predicted molecular weight: 16-32 kDa depending on the level of glycosylation.