Glypican-3 Antibody / GPC3

Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P51654

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 400-550) from the human protein was used as the immunogen for the Glypican-3 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

GPC3/8363R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Glypican-3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Glypican-3 antibody is available for research use only.

Storage Conditions

Aliquot the Glypican-3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Glypican-3 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human placental tissue with Glypican-3 antibody (clone GPC3/8363R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.