Biotin Antibody
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Product Specifications
CAS Number
9000-83-3
Specifications
UniProt
Not Applicable
Host
Mouse
Reactivity
All Species Expected
Immunogen
Biotinylated sheep immunoglobulin was used as the immunogen for the Biotin antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RBTN/8819
Type
Recombinant
Applications
FACS, IF, WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Biotin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Biotin antibody is available for research use only.
Storage Conditions
Aliquot the Biotin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Biotin antibody should be determined by the researcher.
Image Legend
IHC staining of FFPE human kidney tissue with biotinylated Lambda Light Chain antibody probe followed by Biotin antibody (clone rBTN/8819) .
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