Double Stranded DNA Antibody / dsDNA

This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This mAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells.Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Not Applicable

Host

Mouse

Reactivity

Human

Immunogen

Burkitt's cell nuclei were used as the immunogen for the Double Stranded DNA antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG2b κ

Clone

RDSD/8266

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Double Stranded DNA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Double Stranded DNA antibody is available for research use only.

Storage Conditions

Aliquot the Double Stranded DNA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Double Stranded DNA antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human ovarian cancer tissue with Double Stranded DNA antibody (clone rDSD/8266) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.